植物病害分子流行学导论（英）

PART I INTRoDUCTION

　CHAPTER 1 INTRoDUCl’ION TO MoLECULAR EPIDEMIOLoGY

　　1—1 Basic concept of molecular epidemiology

　　1—2 Why molecular epidemiology

　　1—3 Comparisons between classical and molecular methods in epidemiology

　　1—4 Working approaches of molecular epidemiology

　　1—5 About the book

　　References and suggested readings

PARTⅡMoLECULAR TECHNIQUES USED IN EPIDEMIOLOGY

　CHAPTER 2 MOLECULAR MARKERS

　　2—1 Protein—based marker：Allozymes

　　2—2 DNA band—based markers

　　2—3 DNA sequence

　　References and suggested readings

　CltAPTER 3 PCR—BASED TECHNIQUES

　　3—1 Classical PCR

　　3—2 Allele—specific PCR

　　3—3 Nested PCR

　　3—4 Multiplex PCR

　　References and suggested readings

　CHAPTER 4 QUANTITATIVE REAL‘TIME PCR

　　4—1 Introduction

　　4—2 Basic real—time PCR methods

　　4—3 Primer and probe design for real—time PCR

　　4—4 Result analysis

　　4—5 Applications of real—time PCR

　　References and suggested readings

　CHAmR 5 PRIMER I)ESIGN

　　5—1 Some basic concepts for primer design

　　5—2 Computer programs used for primer design

　　5—3 An example of primer design using a computer program

　　5—4 Degenerated PCR primers

PARTⅢ MoLECULAR EPIDEMIOLOGY oF PLANT DISEASES

　CHAPTER 6 PATHoGEN IDENTIFICATIoN AND DISEASE DIAGNoSIS

　　6—1 Introduction

　　6—2 Use of species—specific genes or DNA regions for developing PCR primers

　　6—3 Use of anonymous unique DNA regions for developing PCR primers

　　6—4 PCR inhibitors

　　Summary

　　References and suggested readings

　CHAPTER 7 FUNGAL PATHoGEN REPRoDUCTIVE MoDES

　　7—1 Frequencies of genotypes

　　7—2 Deviation from Hardy—Weinberg expectations

　　7—3 Parsimony Tree Length Permutation(PTLPT)

　　7—4 Index of association(IA)

　　References and suggested readings

　CHAPTER 8 DETEMINATIoN AND QUANTIFICATION OF INITIAL INOCULUM OF EPIDEMICS

　　8—1 Introduction

　　8—2 Molecular methods for quantification of initial inoculum

　　8—3 Examples of quantification of initial inoculum using molecular methods

　　Remarkable conclusion

　　References and suggested readings

　CHPTER 9 TEMpORAL AND SPATIAL DEVELOPMENT oF PATHoGEN POPULATIoNS

　　9—1 Introduction

　　9—2 Molecular approaches

　　9—3 Monitoring pathogen population structures

　　9—4 Studying spatial—temporal dynamics of pathogen populations

　　9—5 Providing information needed for disease management

　　Summary

　　References and suggested readings

　CHAPTER 10 PATHoGEN LONG—DISTANCE DIsPERSAL AND MIGRATION

　　10—1 Introduction

　　10—2 Pathogen lon9—distance dispersal and disease epidemics

　　10—3 Traditional methods to determine or infer pathogen lon9—distance dispersal

　　10—4 Molecular methods to study pathogen lon9—distance dispersal

　　10一5 Examples

　　Summary

　　……

PART IV METHODOLOGY OF DATA ANLYSIS

INDEX